pH-Responsive, Lysine-Based, Hyperbranched Polymers Mimicking Endosomolytic Cell-Penetrating Peptides for Efficient Intracellular Delivery
نویسندگان
چکیده
منابع مشابه
Delivery of short interfering RNA using endosomolytic cell-penetrating peptides.
Cell-penetrating peptides (CPPs) are peptides able to promote uptake of various cargos, including proteins and plasmids. Advances in recent years imply the uptake to be endocytic, where the current hurdle for efficient intracellular delivery is material being retained in the endosomes. In this study we wanted to compare the ability of various established CPPs to deliver siRNA and induce gene si...
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Endosomolytic polymers can aid in the endosomal release of therapeutics to improve intracellular drug delivery. pH-responsive biomimetic pseudo-peptides were synthesised by grafting l-phenylalanine onto the pendant carboxylic acids of a polyamide, poly(l-lysine isophthalamide). PP-75 (stoichiometric l-phenylalanine grafting of 75 mol%) was determined to have the best endosomolytic property. The...
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Small interfering RNA (siRNA) is a promising molecule for gene therapy, but its therapeutic administration remains problematic. Among the recently proposed vectors, cell-penetrating peptides show great promise in in vivo trials for siRNA delivery. Human protein DMBT1 (deleted in malignant brain tumor 1) is a pattern recognition molecule that interacts with polyanions and recognizes and aggregat...
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siRNA has been widely used to identify gene functions and holds great potential in providing a new class of therapeutics.1 The lack of efficient delivery systems is limiting the full therapeutic potential of siRNA technology and represents a challenging hurdle in this field of therapy. For the purpose of nucleic acid transfer, among other synthetic carrier systems, polycations have been applied...
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ژورنال
عنوان ژورنال: Chemistry of Materials
سال: 2017
ISSN: 0897-4756,1520-5002
DOI: 10.1021/acs.chemmater.7b00054